Police in New Lenox, Ill., say two men were killed and three others were injured in the Saturday crash.
Enbridge spokeswoman Lorraine Little said the pipeline was shut down immediately when sensors detected a drop in pressure. A second, undamaged line was also shut down as a precaution.
Both lines carry crude oil from Enbridge's facility in Superior, Wis. to a terminal in Griffith, Ind..
Little said the undamaged line has reopened, but Line 14 remains closed and she said it likely won't be restarted until Thursday.
"Emergency crews and Enbridge crews were at the site as quickly as possible. At this time our thoughts are with the accident victims and their families," Little said on Sunday.